Anti NGAL (rat, neutrophil gelatinase-associated lipocalin), biotinylated
Mouse monoclonal antibody
Cat.No. ABS 039-32B
Preparation: Biotinylated
Content: 50 µL, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.13 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Rat lipocalin-2 is also called rat NGAL as it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL). Rat NGAL is a 25-kDa alpha 2-microglobulin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including cells of the renal proximal tubules. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is upregulated in a variety of pathological situations including infection, inflammation, ischemia-reperfusion and in certain adenocarcinomas.
Recombinant rat NGAL precursor adsorbed onto aluminum hydroxide gel
ABS 039-32 binds rat NGAL and shows no cross-reaction with human NGAL.
Not determined.
ABS 039-32 binds free rat NGAL in solution. ABS 039-32 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 039-08 (as capture antibody) for measuring rat NGAL, giving detection limit of 0.012 ng/mL in an unoptimized buffer assay.
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 1 µg/ml. Users should determine the optimal dilutions for their own purposes.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/4000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
1. Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, Barasch J, Devarajan P (2003) Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol 14:2534-43.
Downloads
ABS 039-32B.pdf (30.54 kB) Product specification
Rat NGAL flyer.pdf (246.86 kB)
Rat NGAL flyer
NGAL rat mouse ref.pdf (360.76 kB)
Rat and mouse NGAL references April 2008
